{"id":46965,"date":"2023-02-03T10:10:01","date_gmt":"2023-02-03T03:10:01","guid":{"rendered":"https:\/\/wiralabanalitika.com\/product\/kolom-kromatografi-cair-bio\/"},"modified":"2023-02-13T10:26:46","modified_gmt":"2023-02-13T03:26:46","slug":"bio-liquid-chromatography-columns","status":"publish","type":"product","link":"https:\/\/wiralabanalitika.com\/en\/product\/bio-liquid-chromatography-columns\/","title":{"rendered":"Bio Liquid Chromatography Columns &#038; Accessories"},"content":{"rendered":"<p><span style=\"font-weight: 400;\">The diversity of biological samples in terms of compound structure and properties coupled with matrix complexity demands a range of sample separation modes, column chemistries, column configurations and detection techniques for their effective characterization. We address these needs with a range of silica and polymeric columns specifically designed to handle the unique rigors of the analysis of proteins, peptides, oligonucleotides and other biomolecules.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">The analysis of proteins, peptides, oligonucleotides and other biomolecules demands a range of sample separation modes, column chemistries, column configurations and detection techniques. Thermo Scientific range of polymeric and silica columns in analytical and nano-scale formats are designed to handle these challenging separations.<\/span><\/p>\n<p><span style=\"color: #003366;\"><b>Liquid Chromatography Columns for Protein\u00a0<\/b><\/span><\/p>\n<p><span style=\"font-weight: 400;\">Reversed Phase:<\/span><\/p>\n<ol>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Thermo Scientific BioBasic\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Acclaim 300 C18 features 3\u03bcm silica particles for rapid analysis of complex protein digests.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Thermo Scientific\u2122 ProSwift\u2122 RP\u00a0<\/span><\/li>\n<\/ol>\n<p><span style=\"font-weight: 400;\">Ion exchange:<\/span><\/p>\n<ol>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Thermo Scientific\u2122 ProPac\u2122 and Thermo Scientific\u2122 MAbPac\u2122 ion exchange columns<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">ProPac WCX and MAbPac SCX columns (for monoclonal separation and analytical characterization). <\/span><i><span style=\"font-weight: 400;\">MAbPac columns are specifically designed for the analysis of monoclonal antibody variants.<\/span><\/i><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">BioBasic AX and BioBasic SCX ion exchange columns<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">ProSwift ion exchange monoliths\u00a0<\/span><\/li>\n<\/ol>\n<p><span style=\"font-weight: 400;\">Size exclusion:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">BioBasic SEC columns<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">MAbPac SEC-1 (300\u00c5 5\u03bcm silica)\u00a0<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">Hydrophobic interaction:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">The ProPac HIC-10<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">ProPac HIC columns\u00a0<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">Affinity<\/span><\/p>\n<p><i><span style=\"font-weight: 400;\">The MAbPac Protein A column<\/span><\/i><span style=\"font-weight: 400;\"> is a unique non-porous polymeric column designed for fast, accurate determination of monoclonal antibody titer analysis from harvest cell culture. <\/span><i><span style=\"font-weight: 400;\">The ProPac IMAC-10<\/span><\/i><span style=\"font-weight: 400;\"> is a high-resolution analytical and semi preparative column for separation of proteins and peptides by immobilized metal affinity chromatography. It is packed with 10\u03bcm, nonporous, polymeric beads coated with a hydrophilic layer, then grafted with poly (IDA) chains.<\/span><\/p>\n<p><i><span style=\"font-weight: 400;\">The ProSwift ConA-1S affinity monolith column<\/span><\/i><span style=\"font-weight: 400;\"> is unsurpassed for fast, highly efficient enrichment and purification of Concanavalin A (Con A) binding glycans, glycopeptides, and glycoproteins containing high-mannose regions.<\/span><\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone wp-image-46691 size-medium\" src=\"http:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-11-200x300.jpg\" alt=\"liquid chromatography column bio\" width=\"200\" height=\"300\" srcset=\"https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-11-200x300.jpg 200w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-11.jpg 341w\" sizes=\"(max-width: 200px) 100vw, 200px\" \/><\/p>\n<p><span style=\"font-weight: 400;\"><strong><span style=\"color: #003366;\">Liquid Chromatography Columns for Oligonucleotides<\/span><\/strong><\/span><\/p>\n<ul>\n<li aria-level=\"1\"><span style=\"font-weight: 400;\">Thermo Scientific DNAPac strong anion exchange columns\u00a0<\/span><\/li>\n<\/ul>\n<ul>\n<li aria-level=\"1\"><span style=\"font-weight: 400;\">Thermo Scientific\u2122 DNASwift\u2122 a strong anion exchange monolith column\u00a0<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\"><strong><span style=\"color: #003366;\">Liquid Chromatography Columns for Carbohydrates<\/span><\/strong><\/span><\/p>\n<p><i><span style=\"font-weight: 400;\">Thermo Scientific\u2122 GlycanPac\u2122 AXH-1<\/span><\/i><span style=\"font-weight: 400;\"> and <\/span><i><span style=\"font-weight: 400;\">AXR-1 columns <\/span><\/i><span style=\"font-weight: 400;\">are HPLC columns designed for the simultaneous separation of glycans by charge, size and polarity. Separating either fluorescently labeled or native glycans.<\/span><\/p>\n<p><span style=\"color: #003366;\"><b>Liquid Chromatography Columns for Proteomics<\/b><\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\">Nano, capillary and micro columns<\/span><\/li>\n<\/ul>\n<p style=\"padding-left: 40px;\"><i><span style=\"font-weight: 400;\">Thermo Scientific\u2122 Acclaim\u2122 PepMap\u2122<\/span><\/i><span style=\"font-weight: 400;\"> and <\/span><i><span style=\"font-weight: 400;\">PepMap RSLC columns<\/span><\/i><span style=\"font-weight: 400;\"> are specially designed for high-resolution analyses of tryptic, natural, and synthetic peptides.\u00a0<\/span><\/p>\n<p style=\"padding-left: 40px;\"><i><span style=\"font-weight: 400;\">Acclaim PepMap Trap columns<\/span><\/i><span style=\"font-weight: 400;\"> are typically applied for the desalting and preconcentration of peptides before LC separation with MS detection.<\/span><\/p>\n<p style=\"padding-left: 40px;\"><i><span style=\"font-weight: 400;\">Thermo Scientific\u2122 PepSwift\u2122 monolithic columns<\/span><\/i><span style=\"font-weight: 400;\"> are specially designed for fast separation and identification of proteins and peptides using nano and capillary LC coupled to MS.<\/span><\/p>\n<p style=\"padding-left: 40px;\"><span style=\"font-weight: 400;\">Using highly pure chromatographic media and biocompatible, metal-free fused silica capillaries, <\/span><i><span style=\"font-weight: 400;\">Thermo Scientific\u2122 EASY-Spray\u2122 capillary LC columns<\/span><\/i><span style=\"font-weight: 400;\"> are produced with a focus on design simplicity and strict quality control.<\/span><\/p>\n<p style=\"padding-left: 40px;\"><i><span style=\"font-weight: 400;\">EASY-Spray columns<\/span><\/i><span style=\"font-weight: 400;\"> provide a fully integrated and temperature controlled combined column emitter design with only a single <\/span><i><span style=\"font-weight: 400;\">Thermo Scientific\u2122 nanoViper\u2122<\/span><\/i><span style=\"font-weight: 400;\"> connection between the LC and the MS ion source.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-weight: 400;\">Moore information on our range of Bio LC columns, visit <\/span><a href=\"http:\/\/thermofisher.com\/biolc\"><span style=\"color: #ff0000;\"><b>thermofisher.com\/biolc<\/b><\/span><\/a><\/p>\n<p>&nbsp;<\/p>\n<ul>\n<li aria-level=\"1\"><span style=\"color: #003366;\"><strong>MAbPac HPLC and UHPLC Columns<\/strong><\/span><\/li>\n<\/ul>\n<p><b>MAbPac Protein A<\/b><\/p>\n<p><span style=\"font-weight: 400;\">Fast mAb titer analysis<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\"> High-efficiency column<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Rugged, long column lifetime<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Excellent sample recovery<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Designed for ease of use and automation<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">Thermo Scientific\u2122 MAbPac\u2122 Protein A is an affinity column designed to provide fast monoclonal antibody (mAb) titer analysis of samples such as harvest cell cultures (HCC).\u00a0 This HPLC column offers high throughput and accurate analysis through a combination of low back pressure and high efficiency.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400;\">The MAbPac Protein A column format allows rapid automation of loading, binding, elution and collection using Thermo Scientific biocompatible systems.<\/span><\/p>\n<p><span style=\"font-weight: 400;\">\u00a0The column is based on a novel non-porous polymeric resin consisting of a divinylbenzene core and a hydrophilic surface, optimized for affinity separation.<\/span><\/p>\n<p><img decoding=\"async\" class=\"alignnone wp-image-46693 size-medium lazyload\" data-src=\"http:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-24-300x186.png\" alt=\"mabpac\" width=\"300\" height=\"186\" data-srcset=\"https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-24-300x186.png 300w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-24-500x310.png 500w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-24.png 512w\" data-sizes=\"(max-width: 300px) 100vw, 300px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 300px; --smush-placeholder-aspect-ratio: 300\/186;\" \/><\/p>\n<p><b>MAbPac SEC-1<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A size exclusion chromatography (SEC) column specifically designed for the high-resolution separation and characterization of monoclonal antibodies (mAbs) and their aggregates.\u00a0<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\"> Analysis of monoclonal antibodies (mAbs) and their aggregates<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Analysis of mAb Fab and Fc fragments, even using high and low salt concentrations<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Hydrophilic bonded layer for minimal undesired interactions between the biomolecules and the stationary phase<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Stable surface bonding leads to low column bleed and compatibility with MS, ELSD and Corona Charged Aerosol Detection (CAD)<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Separation range for globular proteins 10,000\u22121,000,000; exclusion limit for\u00a0 globular proteins &gt;1,000,000<\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" class=\"alignnone size-medium wp-image-46695 lazyload\" data-src=\"http:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-25-300x144.png\" alt=\"kolom bio mabpac sec\" width=\"300\" height=\"144\" data-srcset=\"https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-25-300x144.png 300w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-25-500x240.png 500w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-25.png 512w\" data-sizes=\"(max-width: 300px) 100vw, 300px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 300px; --smush-placeholder-aspect-ratio: 300\/144;\" \/><\/p>\n<p><b>MAbPac SCX-10<\/b><\/p>\n<p><span style=\"font-weight: 400;\">Strong cation exchange column designed specifically for the high-resolution, high efficiency charged variant analysis of monoclonal antibodies and associated variants.<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\"> Exceptionally high-resolution for monoclonal antibody charged variants separation<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Ideal for characterization and quality control assessment of monoclonal antibodies<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Unmatched column-to-column and lot-to-lot reproducibility<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Hydrophobic interactions greatly minimized<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Ideal for stability studies<\/span><\/li>\n<li><span style=\"font-weight: 400;\">Meets the regulatory requirements for biopharmaceutical characterization.<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">Learn more at <\/span><span style=\"color: #ff0000;\"><a style=\"color: #ff0000;\" href=\"http:\/\/thermofisher.com\/mabpac\"><b>thermofisher.com\/mabpac<\/b><\/a><\/span><\/p>\n<p><img decoding=\"async\" class=\"alignnone wp-image-46697 size-medium lazyload\" data-src=\"http:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-26-189x300.png\" alt=\"liquid chormatography column SCX-10\" width=\"189\" height=\"300\" data-srcset=\"https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-26-189x300.png 189w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-26.png 323w\" data-sizes=\"(max-width: 189px) 100vw, 189px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 189px; --smush-placeholder-aspect-ratio: 189\/300;\" \/><\/p>\n<p>&nbsp;<\/p>\n<ul>\n<li aria-level=\"1\"><strong><span style=\"color: #003366;\">Liquid Chromatography Columns for Glycan Analysis<\/span><\/strong><\/li>\n<\/ul>\n<p><b>Accucore 150-amide-HILIC<\/b><\/p>\n<p><span style=\"font-weight: 400;\">HPLC column for neutral glycan analysis<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\"> Amide phase bonded onto 150 \u00c5 pore diameter solid core particles<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> High retention of a broad range of hydrophilic analytes in HILIC mode<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Recommended for hydrophilic bio molecules such as glycans<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">The amide bonded phases provide strong hydrogen bonding interaction between the stationary phase and the analytes, resulting in unique selectivity compared to other HILIC phases. Combined with larger pore size of the solid core particles, Accucore 150-Amide-HILIC is well suited for separating a variety of hydrophilic molecules, including carbohydrates and peptides. As a result, the Accucore 150-Amide-HILIC is an excellent choice for glycan separations.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><b>GlycanPac AXH-1<\/b><\/p>\n<p><span style=\"font-weight: 400;\">High-resolution columns for neutral and charged glycan analysis<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Unique glycan selectivity based on charge, size and polarity<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Excellent resolution for both native and labeled glycans<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Useful for both high-resolution glycan profile characterization and easy quantification of glycans based on charge, size and polarity<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Compatible with fluorescence and MS detection methods<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">\u00a0High chromatographic efficiency and excellent column stability\u00a0<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">Thermo Scientific GlycanPac AXH-1 is a high-performance, silica-based HPLC column for simultaneous separation of glycans by charge, size and polarity. It is designed for high-resolution and high-throughput analysis of biologically important glycans, either labeled or native, by LC-fluorescence and LC-MS methods.<\/span><\/p>\n<p><img decoding=\"async\" class=\"alignnone wp-image-46699 lazyload\" data-src=\"http:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-12-300x107.jpg\" alt=\"liquid chromatography column for glycan\" width=\"311\" height=\"111\" data-srcset=\"https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-12-300x107.jpg 300w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-12-500x178.jpg 500w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-12.jpg 512w\" data-sizes=\"(max-width: 311px) 100vw, 311px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 311px; --smush-placeholder-aspect-ratio: 311\/111;\" \/><\/p>\n<p><b>GlycanPac AXR-1<\/b><\/p>\n<p><span style=\"font-weight: 400;\">Ultra-high resolution columns for neutral, charged and glycan isomer analysis<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\"> Excellent glycan selectivity based on hydrophobicity, charge, size and isomerization<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> High resolution for isomeric glycans<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Compatibility with fluorescence and MS detection methods<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> High column efficiency and stability<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Ideal tool for qualitative, quantitative and structural analysis of glycans\u00a0<\/span><\/li>\n<\/ul>\n<p><img decoding=\"async\" class=\"alignnone wp-image-46701 size-medium lazyload\" data-src=\"http:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-13-298x300.jpg\" alt=\"liquid chromatography glycan axr\" width=\"298\" height=\"300\" data-srcset=\"https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-13-298x300.jpg 298w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-13-150x150.jpg 150w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-13-500x504.jpg 500w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-13-100x100.jpg 100w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-13.jpg 508w\" data-sizes=\"(max-width: 298px) 100vw, 298px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 298px; --smush-placeholder-aspect-ratio: 298\/300;\" \/><\/p>\n<ul>\n<li><span style=\"color: #003366;\"><b>Oligonucleotide Columns<\/b><\/span><\/li>\n<\/ul>\n<p><b>DNAPac PA100<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A strong anion exchange column developed to provide high-resolution analysis and purification of synthetic oligonucleotides<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">\u00a0High-resolution oligonucleotide separations<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">\u00a0Achieve n, n-1 resolution for oligonucleotides<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Resolves oligonucleotides with secondary structures\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">\u00a0Suitable for the analysis of phosphorothioate-based clinical samples<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Easy scale-up from 2.0mm to 22mm ID column (&gt;100x)<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">The DNAPac PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13\u03bcm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized Thermo Scientific\u2122 Dionex\u2122 MicroBeads\u2122. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations. DNAPac PA100 can resolve full length from n\u20131, n+1, and other failure sequences.<\/span><\/p>\n<p><img decoding=\"async\" class=\"alignnone size-medium wp-image-46703 lazyload\" data-src=\"http:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-27-300x209.png\" alt=\"kolom thermo\" width=\"300\" height=\"209\" data-srcset=\"https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-27-300x209.png 300w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-27-500x348.png 500w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-27.png 512w\" data-sizes=\"(max-width: 300px) 100vw, 300px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 300px; --smush-placeholder-aspect-ratio: 300\/209;\" \/><\/p>\n<p><b>DNAPac PA200<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A strong anion exchange column developed to provide high-resolution analysis and purification of synthetic oligonucleotides<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">\u00a0High-resolution oligonucleotide separations<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">\u00a0Achieve n, n-1 resolution for oligonucleotides<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Resolves oligonucleotides with secondary structures\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">\u00a0Suitable for the analysis of phosphorothioate-based clinical samples<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">Easy scale-up from 2.0mm to 22mm ID column (&gt;100x)<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">The DNAPac PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13\u03bcm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized Thermo Scientific\u2122 Dionex\u2122 MicroBeads\u2122. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations. DNAPac PA100 can resolve full length from n\u20131, n+1, and other failure sequences.<\/span><\/p>\n<p><img decoding=\"async\" class=\"alignnone size-medium wp-image-46705 lazyload\" data-src=\"http:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/F176509_peps-650-transformed-300x202.png\" alt=\"kolom peps\" width=\"300\" height=\"202\" data-srcset=\"https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/F176509_peps-650-transformed-300x202.png 300w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/F176509_peps-650-transformed-500x336.png 500w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/F176509_peps-650-transformed-600x403.png 600w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/F176509_peps-650-transformed.png 650w\" data-sizes=\"(max-width: 300px) 100vw, 300px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 300px; --smush-placeholder-aspect-ratio: 300\/202;\" \/><\/p>\n<p><b>DNAPac PA200 RS<\/b><\/p>\n<p><span style=\"font-weight: 400;\">UHPLC Solutions for nucleic acid analysis<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\"> Provide single base resolution of oligonucleotides\u00a0<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Higher efficiency to improve resolution<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Improved throughput<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Ruggedness consistent with the DNAPac PA200 column line<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Stable to 10,000 psi<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">DNAPac PA200 RS columns are packed with smaller, 4\u03bcm particles, for improved resolution and better performance. The smaller particles also allow shorter columns to provide significantly higher throughput. These columns are packed in Bio-Inert PEEK-lined stainless steel (SST) bodies, designed to protect from unwanted interactions of eluents and analytes with metals, while maintaining 10,000 psi stability. These columns offer exceptional resolution of oligonucleotides, including isomer separations; and are able to resolve full length oligonucleotides from n-1 and n+1 oligonucleotides and other failure sequences.<\/span><\/p>\n<p><img decoding=\"async\" class=\"alignnone size-medium wp-image-46707 lazyload\" data-src=\"http:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-28-300x149.png\" alt=\"kolom dnapac\" width=\"300\" height=\"149\" data-srcset=\"https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-28-300x149.png 300w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-28-500x249.png 500w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-28.png 512w\" data-sizes=\"(max-width: 300px) 100vw, 300px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 300px; --smush-placeholder-aspect-ratio: 300\/149;\" \/><\/p>\n<p><b>DNASwift SAX-1S<\/b><\/p>\n<p><span style=\"font-weight: 400;\">A strong anion exchange monolith column that provides improved capacity and industry-leading oligonucleotide yield-purity performance.<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\"> Micromole purifications in a 5mm ID column body<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Substantial capacity in a small format\u00a0<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Tunable selectivity control, like the DNAPac PA200 columns, for high resolution<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Compatible with high pH mobile phases, solvents, or high temperatures<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Ideal for therapeutic and diagnostic oligonucleotide research<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Purify difficult oligonucleotide products<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">The DNASwift column is a unique porous polymer monolith coated with functionalized latex nanobeads, optimized for oligonucleotide separations. The monolith, a polymer cylinder with interconnected flow through channels, provides fast mass transfer, low back pressure (for increased flow rates), very high capacity, and refined selectivity control.<\/span><\/p>\n<p><img decoding=\"async\" class=\"alignnone size-medium wp-image-46709 lazyload\" data-src=\"http:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-29-300x150.png\" alt=\"kolom dna\" width=\"300\" height=\"150\" data-srcset=\"https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-29-300x150.png 300w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-29-500x250.png 500w, https:\/\/wiralabanalitika.com\/wp-content\/uploads\/2023\/02\/unnamed-29.png 512w\" data-sizes=\"(max-width: 300px) 100vw, 300px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 300px; --smush-placeholder-aspect-ratio: 300\/150;\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><b>DNAPac RP<\/b><\/p>\n<p><span style=\"font-weight: 400;\">Reverse phase (RP) column specifically for analysis of oligonucleotides and double stranded (ds) DNA and RNA fragments.<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400;\"> Excellent MS compatibility<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> Wide operating pH range: 0\u201314<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> High temperature stability: up to 100\u00b0C<\/span><\/li>\n<li><span style=\"font-weight: 400;\"> High throughput<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400;\">The DNAPac RP is a column designed for analysis of oligonucleotides and ds DNA and RNA fragments using LC\/UV or LC\/MS. The unique column chemistry provides excellent performance under a broad range of pH, temperature, and mobile phase compositions. In addition, the large pore size of the resin provides excellent separation of small to large oligonucleotides.<\/span><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-weight: 400;\">Learn more at <\/span><a href=\"http:\/\/thermofisher.com\/LCcolumns\"><span style=\"color: #ff0000;\"><b>thermofisher.com\/LCcolumns<\/b><\/span><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The diversity of biological samples in terms of compound structure and properties coupled with matrix complexity demands a range of sample separation modes, column chemistries, column configurations and detection techniques for their effective characterization. We address these needs with a range of silica and polymeric columns specifically designed to handle the unique rigors of the [&hellip;]<\/p>\n","protected":false},"featured_media":46692,"comment_status":"open","ping_status":"closed","template":"","meta":{"_acf_changed":false},"product_brand":[],"product_cat":[77,125,88,76],"product_tag":[],"class_list":{"0":"post-46965","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-application","7":"product_cat-chemicals","8":"product_cat-gc-hplc-and-spe-columns-and-consumable","9":"product_cat-product","11":"first","12":"instock","13":"shipping-taxable","14":"product-type-simple"},"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v25.0 (Yoast SEO v27.4) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Bio Liquid Chromatography Columns &amp; Accessories - Wiralab<\/title>\n<meta name=\"description\" content=\"Liquid chromatography columns that are adapted to the structure and properties of the compounds, and the matrix of the sample biological compounds\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/wiralabanalitika.com\/en\/product\/bio-liquid-chromatography-columns\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Bio Liquid Chromatography Columns &amp; 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