The diversity of biological samples in terms of compound structure and properties coupled with matrix complexity demands a range of sample separation modes, column chemistries, column configurations and detection techniques for their effective characterization. We address these needs with a range of silica and polymeric columns specifically designed to handle the unique rigors of the analysis of proteins, peptides, oligonucleotides and other biomolecules.
The analysis of proteins, peptides, oligonucleotides and other biomolecules demands a range of sample separation modes, column chemistries, column configurations and detection techniques. Thermo Scientific range of polymeric and silica columns in analytical and nano-scale formats are designed to handle these challenging separations.
Liquid Chromatography Columns for ProteinÂ
Reversed Phase:
Ion exchange:
Size exclusion:
Hydrophobic interaction:
Affinity
The MAbPac Protein A column is a unique non-porous polymeric column designed for fast, accurate determination of monoclonal antibody titer analysis from harvest cell culture. The ProPac IMAC-10 is a high-resolution analytical and semi preparative column for separation of proteins and peptides by immobilized metal affinity chromatography. It is packed with 10μm, nonporous, polymeric beads coated with a hydrophilic layer, then grafted with poly (IDA) chains.
The ProSwift ConA-1S affinity monolith column is unsurpassed for fast, highly efficient enrichment and purification of Concanavalin A (Con A) binding glycans, glycopeptides, and glycoproteins containing high-mannose regions.
Liquid Chromatography Columns for Oligonucleotides
Liquid Chromatography Columns for Carbohydrates
Thermo Scientificâ„¢ GlycanPacâ„¢ AXH-1 and AXR-1 columns are HPLC columns designed for the simultaneous separation of glycans by charge, size and polarity. Separating either fluorescently labeled or native glycans.
Liquid Chromatography Columns for Proteomics
Thermo Scientificâ„¢ Acclaimâ„¢ PepMapâ„¢ and PepMap RSLC columns are specially designed for high-resolution analyses of tryptic, natural, and synthetic peptides.Â
Acclaim PepMap Trap columns are typically applied for the desalting and preconcentration of peptides before LC separation with MS detection.
Thermo Scientificâ„¢ PepSwiftâ„¢ monolithic columns are specially designed for fast separation and identification of proteins and peptides using nano and capillary LC coupled to MS.
Using highly pure chromatographic media and biocompatible, metal-free fused silica capillaries, Thermo Scientificâ„¢ EASY-Sprayâ„¢ capillary LC columns are produced with a focus on design simplicity and strict quality control.
EASY-Spray columns provide a fully integrated and temperature controlled combined column emitter design with only a single Thermo Scientificâ„¢ nanoViperâ„¢ connection between the LC and the MS ion source.
Moore information on our range of Bio LC columns, visit thermofisher.com/biolc
MAbPac Protein A
Fast mAb titer analysis
Thermo Scientificâ„¢ MAbPacâ„¢ Protein A is an affinity column designed to provide fast monoclonal antibody (mAb) titer analysis of samples such as harvest cell cultures (HCC). This HPLC column offers high throughput and accurate analysis through a combination of low back pressure and high efficiency.Â
The MAbPac Protein A column format allows rapid automation of loading, binding, elution and collection using Thermo Scientific biocompatible systems.
 The column is based on a novel non-porous polymeric resin consisting of a divinylbenzene core and a hydrophilic surface, optimized for affinity separation.
MAbPac SEC-1
A size exclusion chromatography (SEC) column specifically designed for the high-resolution separation and characterization of monoclonal antibodies (mAbs) and their aggregates.Â
MAbPac SCX-10
Strong cation exchange column designed specifically for the high-resolution, high efficiency charged variant analysis of monoclonal antibodies and associated variants.
Learn more at thermofisher.com/mabpac
Accucore 150-amide-HILIC
HPLC column for neutral glycan analysis
The amide bonded phases provide strong hydrogen bonding interaction between the stationary phase and the analytes, resulting in unique selectivity compared to other HILIC phases. Combined with larger pore size of the solid core particles, Accucore 150-Amide-HILIC is well suited for separating a variety of hydrophilic molecules, including carbohydrates and peptides. As a result, the Accucore 150-Amide-HILIC is an excellent choice for glycan separations.
GlycanPac AXH-1
High-resolution columns for neutral and charged glycan analysis
Thermo Scientific GlycanPac AXH-1 is a high-performance, silica-based HPLC column for simultaneous separation of glycans by charge, size and polarity. It is designed for high-resolution and high-throughput analysis of biologically important glycans, either labeled or native, by LC-fluorescence and LC-MS methods.
GlycanPac AXR-1
Ultra-high resolution columns for neutral, charged and glycan isomer analysis
DNAPac PA100
A strong anion exchange column developed to provide high-resolution analysis and purification of synthetic oligonucleotides
The DNAPac PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized Thermo Scientific™ Dionex™ MicroBeads™. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations. DNAPac PA100 can resolve full length from n–1, n+1, and other failure sequences.
DNAPac PA200
A strong anion exchange column developed to provide high-resolution analysis and purification of synthetic oligonucleotides
The DNAPac PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized Thermo Scientific™ Dionex™ MicroBeads™. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations. DNAPac PA100 can resolve full length from n–1, n+1, and other failure sequences.
DNAPac PA200 RS
UHPLC Solutions for nucleic acid analysis
DNAPac PA200 RS columns are packed with smaller, 4μm particles, for improved resolution and better performance. The smaller particles also allow shorter columns to provide significantly higher throughput. These columns are packed in Bio-Inert PEEK-lined stainless steel (SST) bodies, designed to protect from unwanted interactions of eluents and analytes with metals, while maintaining 10,000 psi stability. These columns offer exceptional resolution of oligonucleotides, including isomer separations; and are able to resolve full length oligonucleotides from n-1 and n+1 oligonucleotides and other failure sequences.
DNASwift SAX-1S
A strong anion exchange monolith column that provides improved capacity and industry-leading oligonucleotide yield-purity performance.
The DNASwift column is a unique porous polymer monolith coated with functionalized latex nanobeads, optimized for oligonucleotide separations. The monolith, a polymer cylinder with interconnected flow through channels, provides fast mass transfer, low back pressure (for increased flow rates), very high capacity, and refined selectivity control.
DNAPac RP
Reverse phase (RP) column specifically for analysis of oligonucleotides and double stranded (ds) DNA and RNA fragments.
The DNAPac RP is a column designed for analysis of oligonucleotides and ds DNA and RNA fragments using LC/UV or LC/MS. The unique column chemistry provides excellent performance under a broad range of pH, temperature, and mobile phase compositions. In addition, the large pore size of the resin provides excellent separation of small to large oligonucleotides.
Learn more at thermofisher.com/LCcolumns
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