Solid Phase Extraction Dionex™ AutoTrace™ 280


Solid Phase Extraction Dionex™ AutoTrace™ 280

The Dionex AutoTrace 280 instrument is an automated solid phase extraction (SPE) system for use with large samples (20 mL–20 L) for the isolation of trace organics in water or aqueous matrices. The compounds of interest are trapped on SPE adsorbents (cartridge or disk format) then eluted with strong solvents to generate an extract ready for analysis.

The Dionex AutoTrace 280™ instrument saves time, solvent, and labor ensuring high reproducibility and productivity for analytical laboratories. The instrument can process up to 6 samples in 2–3 hours with only 15 minutes of operator involvement. The Dionex AutoTrace 280 instrument uses powerful pumps (no check valves) and proven constant-flow technology to efficiently process even the most difficult samples. With Dionex AutoTrace and Thermo Scientific Dionex Accelerated Solvent Extraction (ASE) systems, laboratories can effectively automate the solvent-extraction process for liquid and solid matrices.


Instrument Operation

The Dionex AutoTrace 280 instrument automates the solid phase extraction process. First, the sample cartridges or disks are conditioned with solvent or buffer. Next, the liquid or water samples are pumped from the sample container through the SPE cartridges or discs. As the sample passes through the SPE material, analytes of interest are adsorbed and the liquid goes to aqueous waste. Then, the SPE material is rinsed to remove possible interference. Finally, the analytes of interest are eluted from the SPE material with a strong solvent and collected.



  • Automated extraction for liquid sample sizes of 20 mL –    4 L
  • Dramatic reduction in solvent usage and reduced sample handling
  • Approved for use by many government agencies
  • Extraction of six channels simultaneously


Applications and Systems Performance

Current analytical methods that may require SPE preparation include GC, GC-MS, LC, and LC-MS, and cover the following sample matrices:

  • Pesticides (OCPs, OPPs, diquats, and urea ionic pesticides)
  • Pollutants (phenols, PCBs, nitrosamines, and dioxins)
  • Personal care products (pharmaceuticals, steroids, and endocrine disruptors)
  • Total petroleum hydrocarbons (DRO)
  • Explosive residues
  • Beverages and flavor components

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